The FGFR3 Polyclonal Antibody (CAB24846) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the FGFR3 protein, researchers can easily detect and analyze its expression in various cell types, making it an excellent choice for studies in developmental biology and cancer research.FGFR3 plays a crucial role in cell signaling pathways that govern cell proliferation, differentiation, and survival.
This antibody is validated for use in WB, FC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
FGFR3 Polyclonal Antibody
SKU:
CAB24846
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Membrane, Cytoplasmic Vesicle, Endoplasmic Reticulum, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
75kDa/85kDa/87kDa/88kDa
Observed MW:
100-150kDa
This gene encodes a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia. Three alternatively spliced transcript variants that encode different protein isoforms have been described.
Purification Method
Affinity purification
Gene ID
2261
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from K-562 cells using [KD Validated] FGFR3 Rabbit pAb (CAB24846) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and FGFR3 knockdown (KD) 293T cells using [KD Validated] FGFR3 Rabbit pAb (CAB24846) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
