The FGL2 Antibody (CAB13755) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed through rabbit immunization, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the FGL2 protein, this antibody enables the detection and analysis of FGL2 in a variety of cell types, making it an ideal choice for studies in immunology and hemostasis research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
FGL2 Antibody
SKU:
CAB13755
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
NNET EEIK DERA KDVC PVRL ESRG KCEE AGEC PYQV SLPP LTIQ LPKQ FSRI EEVF KEVQ NLKE IVNS LKKS CQDC KLQA DDNG DPGR NGLL LPST GAPG EVGD NRVR ELES EVNK L
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FGL2, T49, pT49
Positive Sample:
HepG2
Cellular Localization:
Secreted.
Calculated MW:
50kDa
Observed MW:
70kDa
The protein encoded by this gene is a secreted protein that is similar to the beta- and gamma-chains of fibrinogen. The carboxyl-terminus of the encoded protein consists of the fibrinogen-related domains (FRED). The encoded protein forms a tetrameric complex which is stabilized by interchain disulfide bonds. This protein may play a role in physiologic functions at mucosal sites.
Purification Method
Affinity purification
Gene ID
10875
RRID
AB_2760612
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using FGL2 Rabbit pAb (CAB13755) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
