The FHL5 Antibody (CAB15756) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with both human and mouse samples, making it versatile for use in various experimental applications. Specifically validated for use in Western blotting, the FHL5 Polyclonal Antibody binds to the FHL5 protein, allowing for precise detection and analysis in different cell types.FHL5, also known as Four and a Half LIM Domains protein 5, is a transcription factor that plays a role in gene expression regulation and tumor suppression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
FHL5 Antibody
SKU:
CAB15756
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACT, FHL-5, dJ393D12.2, 1700027G07Rik, FHL5
Positive Sample:
DU145, PC-3, U-87MG, Mouse heart
Cellular Localization:
Nucleus.
Calculated MW:
33kDa
Observed MW:
33kDa
The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. Polymorphisms in this gene may be associated with susceptibility for migraine headaches. Alternative splicing results in multiple transcript variants encoding the same protein.
Purification Method
Affinity purification
Gene ID
9457
RRID
AB_2763173
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FHL5 Rabbit pAb (CAB15756) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
