The FKBP52/FKBP4 Antibody (CAB5643) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity when used in Western blot applications with human samples. By targeting the FKBP4 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for investigations in molecular biology and drug development.FKBP4, also known as FK506-binding protein 4, plays a crucial role in modulating protein folding and stability, making it a potential therapeutic target for various diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FKBP52/FKBP4 Antibody
SKU:
CAB5643
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene.
Purification Method
Affinity purification
Gene ID
2288
RRID
AB_2766403
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FKBP52/FKBP4 Rabbit pAb (CAB5643) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using FKBP52/FKBP4 Rabbit pAb (CAB5643) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg FKBP52/FKBP52/FKBP4 antibody (CAB5643). Western blot was performed from the immunoprecipitate using FKBP52/FKBP52/FKBP4 antibody (CAB5643) at a dilution of 1:1000.
