The FMN (Flavin Mononucleotide) ELISA Kit is an innovative assay designed for the precise and quantitative detection of Flavin Mononucleotide (FMN) levels in biological samples. FMN, a crucial cofactor in various metabolic processes, is essential for critical cellular functions, including energy metabolism and enzymatic reactions. This ELISA kit provides a valuable tool for researchers to explore FMN concentrations and unravel its role in metabolic pathways and redox reactions. Scientific investigators and researchers can leverage the FMN ELISA Kit to accurately measure FMN levels, gaining valuable insights into cellular energy transfer and enzymatic reactions involving FMN.
With exceptional sensitivity and specificity, this kit ensures reliable and reproducible results, facilitating in-depth studies of FMN-related processes and metabolic pathways. Manufactured under stringent quality control standards, the FMN ELISA Kit delivers robust performance and user-friendly operation, making it an ideal choice for metabolic research, enzymology studies, and investigations of flavin-dependent pathways. Uncover the intricate role of FMN in cellular metabolism and energy pathways with this advanced ELISA kit.
Product Name:
FMN (Flavin mononucleotide) ELISA Kit
SKU:
AEES00665
Target:
FMN (Flavin mononucleotide)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with FMN. During the reaction, FMN in the sample or standard competes with a fixed amount of FMN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to FMN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FMN in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
96-109
90-104
Average (%)
99
102
97
1:4
Range (%)
90-104
94-111
90-102
Average (%)
98
101
97
1:8
Range (%)
92-108
95-107
88-99
Average (%)
100
101
93
1:16
Range (%)
91-105
98-110
87-100
Average (%)
97
104
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-106
97
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
93-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.07
150.26
353.12
49.95
141.68
339.22
Standard deviation
2.97
8.61
12.64
3.25
6.89
12.45
C V (%)
5.49
5.73
3.58
6.51
4.86
3.67
Sample type &Sample volume:
; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of FMN concentrations in .
Specificity:
This kit recognizes FMN in samples. No significant cross-reactivity or interference between FMN and analogues was observed.
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