The FMRP Antibody (CAB6092) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it suitable for use in Western blot applications.FMR1 is important for brain development and functioning, and mutations in this gene are linked to Fragile X syndrome, the most common inherited form of intellectual disability. The FMR1 Polyclonal Antibody binds specifically to the FMR1 protein, enabling accurate detection and analysis in various cell types.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FMRP Antibody
SKU:
CAB6092
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
POF, FMRP, POF1, FRAXA
Positive Sample:
293T, C2C12, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Nucleus, Nucleolus.
Calculated MW:
71kDa
Observed MW:
60-80kDa
The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene.
Purification Method
Affinity purification
Gene ID
2332
RRID
AB_2766744
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using FMRP Rabbit pAb (CAB6092) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat lung using FMRP Rabbit pAb (CAB6092) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using FMRP Rabbit pAb (CAB6092) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using FMRP Rabbit pAb (CAB6092) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
