This kit is comprised by HRP conjugate, other auxiliary reagents and ELISA Microtiter plate pre-coated with recombinant Foot and Mouth Disease Virus Type O (FMD-O) protein. Apply the principle of enzyme-linked immunoassay (ELISA) to detect porcine FMD-O antibody in serum of swine, cattle, goat, and sheep. During the experiment, add control serum and samples into the ELISA Microtiter plate. If FMD-O antibodies exist in the samples, it will be bound with the recombinant protein on the microtiter plate after incubation. Then wash the plate to remove unbound antibodies and other components, add the HRP conjugate to specifically bind with the compound of antibody and antigen on the microtiter plate. The unbound HRP conjugate will be removed by washing. Substrate Reagent is added into the well, it will react with the enzyme and the products become blue. The color shade is of negative correlation with antibody levels in the samples. At last, end the reaction by adding stop solution to produce a yellow product. Measure the absorbance value of each well by using a microtiter plate Reader with 450 nm wavelength, then we can know whether there are FMD-O antibody in the samples.
