The FOXJ1 Polyclonal Antibody (CAB23024) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high reactivity with human samples and has been validated for use in various applications, including Western blotting. By specifically binding to the FOXJ1 protein, this antibody allows for the accurate detection and analysis of FOXJ1 expression in a wide range of cell types.FOXJ1 is a key regulator of cilia development and function, playing a crucial role in processes such as mucociliary clearance in the respiratory tract and left-right axis establishment during embryonic development.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
FOXJ1 Polyclonal Antibody
SKU:
CAB23024
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HFH4, HFH-4, CILD43, FKHL13, FOXJ1
Positive Sample:
Mouse brain,
Cellular Localization:
Cell Membrane, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
45kDa
Observed MW:
55kDa
This gene encodes a member of the forkhead family of transcription factors. Similar genes in zebrafish and mouse have been shown to regulate the transcription of genes that control the production of motile cilia. The mouse ortholog also functions in the determination of left-right asymmetry. Polymorphisms in this gene are associated with systemic lupus erythematosus and allergic rhinitis.
Purification Method
Affinity purification
Gene ID
2302
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain using FOXJ1 Rabbit pAb (CAB23024) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time:10s.
Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug FOXJ1 Rabbit pAb (CAB23024 1:90). Western blot was performed from the immunoprecipitate using FOXJ1 Rabbit pAb (CAB23024) at a dilition of 1:3000.
