The FOXL2 Antibody (CAB16244) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human and mouse samples and has been validated for use in various experimental techniques, including Western blot and immunohistochemistry.FOXl2 is a key regulator of granulosa cell differentiation and folliculogenesis, making it a vital player in reproductive biology and ovarian health. Dysregulation of FOXl2 has been implicated in ovarian disorders and reproductive cancers, highlighting its significance in clinical research and therapeutic development.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FOXL2 Antibody
SKU:
CAB16244
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BPES, PFRK, POF3, BPES1, PINTO, FOXL2
Positive Sample:
OVCAR3, PC-3
Cellular Localization:
Nucleus.
Calculated MW:
39kDa
Observed MW:
50kDa
This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Expansion of a polyalanine repeat region and other mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3.
Purification Method
Affinity purification
Gene ID
668
RRID
AB_2769513
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using (CAB16244) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded rat ovary using FOXL2 Rabbit pAb (CAB16244) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
