The FOXP3 Monoclonal Antibody (CAB4953) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
FOXP3 Monoclonal Antibody
SKU:
CAB4953
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC1159
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:1000 - 1:4000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
JM2, AIID, IPEX, PIDX, XPID, DIETER, FOXP3
Positive Sample:
Jurkat
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
47kDa
Observed MW:
47kDa
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method
Affinity purification
Gene ID
50943
RRID
AB_2863396
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using FOXP3 Rabbit mAb (CAB4953) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg FOXP3 antibody (CAB4953). Western blot was performed from the immunoprecipitate using FOXP3 antibody (CAB4953) at a dilution of 1:1000.
