The FOXP3 Monoclonal Antibody (CAB4953) is a high-quality antibody developed for reliable detection and analysis of target proteins. Foxp3, also known as forkhead box P3, is a key transcription factor that plays a critical role in the development and function of regulatory T cells (Tregs). This antibody, generated in rabbits, is highly specific and reacts with human samples, making it suitable for a variety of research applications including immunohistochemistry, flow cytometry, and Western blotting.Tregs are a subset of T cells that play a crucial role in maintaining immune tolerance and preventing autoimmunity. Dysregulation of Tregs and Foxp3 expression has been implicated in a variety of autoimmune diseases, inflammatory conditions, and cancer.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
FOXP3 Monoclonal Antibody
SKU:
CAB4953
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1159
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
JM2, AIID, IPEX, PIDX, XPID, DIETER, FOXP3
Positive Sample:
Jurkat
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
47kDa
Observed MW:
47kDa
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method
Affinity purification
Gene ID
50943
RRID
AB_2863396
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using FOXP3 Rabbit mAb (CAB4953) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg FOXP3 antibody (CAB4953). Western blot was performed from the immunoprecipitate using FOXP3 antibody (CAB4953) at a dilution of 1:1000.
