The FSTL4 Antibody (CAB18233) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting FSTL4 in human samples, making it suitable for use in Western blot applications.FSTL4, also known as follistatin-like 4, has been implicated in both normal development and disease progression, particularly in cancer. By targeting FSTL4, researchers can gain valuable insights into its role in signaling pathways and disease mechanisms, opening up avenues for potential therapeutic interventions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FSTL4 Antibody
SKU:
CAB18233
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FSTL4
Positive Sample:
U-87MG, U-251MG, SH-SY5Y, NCI-H460, A-549, Mouse heart, Mouse brain, Rat heart, Rat brain
Cellular Localization:
Extracellular Region.
Calculated MW:
93kDa
Observed MW:
85kDa
Predicted to enable brain-derived neurotrophic factor binding activity and calcium ion binding activity. Predicted to be involved in cell differentiation. Predicted to act upstream of or within negative regulation of brain-derived neurotrophic factor receptor signaling pathway; negative regulation of collateral sprouting; and negative regulation of dendritic spine development. Predicted to be located in extracellular region and secretory granule.
Purification Method
Affinity purification
Gene ID
23105
RRID
AB_2862009
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FSTL4 Rabbit pAb (CAB18233) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 2s.
Immunofluorescence analysis of L929 cells using FSTL4 Rabbit pAb (CAB18233) at dilution of 1:100. Blue: DAPI for nuclear staining.
