The FSTL4 Antibody (CAB18233) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable brain-derived neurotrophic factor binding activity and calcium ion binding activity. Predicted to be involved in cell differentiation. Predicted to act upstream of or within negative regulation of brain-derived neurotrophic factor receptor signaling pathway; negative regulation of collateral sprouting; and negative regulation of dendritic spine development. Predicted to be located in extracellular region and secretory granule. RRID AB_2862009 Gene ID 23105 Swiss Prot Synonym FSTL4
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FSTL4 Antibody
SKU:
CAB18233
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
-
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FSTL4
Positive Sample:
U-87MG, U-251MG, SH-SY5Y, NCI-H460, A-549, Mouse heart, Mouse brain, Rat heart, Rat brain
Cellular Localization:
Extracellular Region.
Calculated MW:
93kDa
Observed MW:
85kDa
Predicted to enable brain-derived neurotrophic factor binding activity and calcium ion binding activity. Predicted to be involved in cell differentiation. Predicted to act upstream of or within negative regulation of brain-derived neurotrophic factor receptor signaling pathway; negative regulation of collateral sprouting; and negative regulation of dendritic spine development. Predicted to be located in extracellular region and secretory granule. RRID AB_2862009 Gene ID 23105 Swiss Prot Synonym FSTL4
Purification Method:
Affinity purification
Gene ID:
23105
RRID:
AB_2862009
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FSTL4 Rabbit pAb (CAB18233) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 2s.
Immunofluorescence analysis of L929 cells using FSTL4 Rabbit pAb (CAB18233) at dilution of 1:100. Blue: DAPI for nuclear staining.
