The FTO Antibody (CAB1438) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the FTO protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in the fields of molecular biology and metabolic research.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FTO Antibody
SKU:
CAB1438
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GDFD, ALKBH9, BMIQ14, FTO
Positive Sample:
HeLa, 293T, SH-SY5Y
Cellular Localization:
Nucleus, Nucleus Speckle.
Calculated MW:
58kDa
Observed MW:
60kDa
This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes.
Purification Method
Affinity purification
Gene ID
79068
RRID
AB_2761247
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FTO Rabbit pAb (CAB1438) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunoprecipitation analysis of 300 μg extracts of SH-SY5Y cells using 3 μg FTO antibody (CAB1438). Western blot was performed from the immunoprecipitate using FTO antibody (CAB1438) at a dilution of 1:1000.
