The FTO Antibody (CAB1438) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. RRID AB_2761247 Gene ID 79068 Swiss Prot Synonym GDFD; ALKBH9; BMIQ14; FTO
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FTO Antibody
SKU:
CAB1438
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GDFD, ALKBH9, BMIQ14, FTO
Positive Sample:
HeLa, 293T, SH-SY5Y
Cellular Localization:
Nucleus, Nucleus Speckle.
Calculated MW:
58kDa
Observed MW:
60kDa
This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. RRID AB_2761247 Gene ID 79068 Swiss Prot Synonym GDFD; ALKBH9; BMIQ14; FTO
Purification Method:
Affinity purification
Gene ID:
79068
RRID:
AB_2761247
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FTO Rabbit pAb (CAB1438) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunoprecipitation analysis of 300 μg extracts of SH-SY5Y cells using 3 μg FTO antibody (CAB1438). Western blot was performed from the immunoprecipitate using FTO antibody (CAB1438) at a dilution of 1:1000.
