The FTO Antibody (CAB18672) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable 2-oxoglutarate-dependent dioxygenase activity and ferrous iron binding activity. Acts upstream of or within several processes, including cilium assembly; left/right pattern formation; and positive regulation of canonical Wnt signaling pathway. Predicted to be located in cytoplasm and nuclear speck. Human ortholog(s) of this gene implicated in obesity. Orthologous to human FTO (FTO alpha-ketoglutarate dependent dioxygenase).
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FTO Antibody
SKU:
CAB18672
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
Mouse brain, Rat brain
Observed MW:
60kDa
Predicted to enable 2-oxoglutarate-dependent dioxygenase activity and ferrous iron binding activity. Acts upstream of or within several processes, including cilium assembly; left/right pattern formation; and positive regulation of canonical Wnt signaling pathway. Predicted to be located in cytoplasm and nuclear speck. Human ortholog(s) of this gene implicated in obesity. Orthologous to human FTO (FTO alpha-ketoglutarate dependent dioxygenase).
Purification Method
Affinity purification
Gene ID
553363
RRID
AB_2862408
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FTO Rabbit pAb (CAB18672) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
