Description
Furin Antibody (CAB7445)
The Furin Antibody (CAB7445) is a high-quality antibody developed for reliable detection and analysis of target proteins. Furin is a protease enzyme involved in the processing of precursor proteins into their active forms, making it a crucial player in various cellular processes. This antibody, generated in rabbits, is optimized for use in Western blot and immunohistochemistry applications, allowing for precise detection and localization of furin in cell and tissue samples.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | Furin Antibody |
| SKU: | CAB7445 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||||
| Sequence: | GEVA AVAN NGVC GVGV AYNA RIGG VRML DGEV TDAV EARS LGLN PNHI HIYS ASWG PEDD GKTV DGPA RLAE EAFF RGVS QGRG GLGS IFVW ASGN GGRE H | ||||||
| Tested Applications: | WB IF/ICC ELISA | ||||||
| Recommended Dilution: |
| ||||||
| Synonyms: | FUR, PACE, SPC1, PCSK3, Furin |
| Positive Sample: | 293F, Rat liver |
| Cellular Localization: | Cell Membrane, Golgi Apparatus, Single-Pass Type I Membrane Protein, Trans-Golgi Network Membrane. |
| Calculated MW: | 87kDa |
| Observed MW: | 90-110kDa |
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. Like other members of this convertase family, the product of this gene specifically cleaves substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140, and may play a role in tumor progression. Unlike SARS-CoV and other coronaviruses, the spike protein of SARS-CoV-2 is thought to be uniquely cleaved by this protease. Alternative splicing results in multiple transcript variants.
| Purification Method | Affinity purification |
| Gene ID | 5045 |
| RRID | AB_2767976 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from 293F cells, using Furin Rabbit pAb (CAB7445) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
 | Western blot analysis of lysates from Rat liver, using Furin Rabbit pAb (CAB7445) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. |
