The FUT3 Antibody (CAB15058) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit hosts, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the FUT3 protein, researchers can effectively detect and analyze FUT3 expression in various cell types, making it an ideal choice for investigations in glycobiology and cancer research.FUT3, also known as fucosyltransferase 3, plays a crucial role in the modification of glycoproteins and glycolipids, impacting diverse biological processes such as cell adhesion, migration, and signaling.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
FUT3 Antibody
SKU:
CAB15058
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LE, Les, FT3B, CD174, FucT-III, FUT3
Positive Sample:
A-549, HT-29, 293T, LO2
Cellular Localization:
Golgi Apparatus, Golgi Stack Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
42kDa
Observed MW:
50kDa
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Differences in the expression of this gene are associated with host susceptibility to viral infection.
Purification Method
Affinity purification
Gene ID
2525
RRID
AB_2761939
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FUT3 Rabbit pAb (CAB15058) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
