The FUT6 Antibody (CAB3868) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By binding to the FUT6 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in glycobiology and cancer research.FUT6, a fucosyltransferase enzyme, plays a critical role in the modification of glycoproteins and glycolipids with fucose residues, which are essential for various cellular processes such as cell-cell interactions, signaling, and immune response regulation.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
FUT6 Antibody
SKU:
CAB3868
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FT1A, FCT3A, Fuc-TVI, FucT-VI, FUT6
Positive Sample:
SW480
Cellular Localization:
Golgi Apparatus, Golgi Stack Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
42kDa
Observed MW:
42kDa
The protein encoded by this gene is a Golgi stack membrane protein that is involved in the creation of sialyl-Lewis X, an E-selectin ligand. Mutations in this gene are a cause of fucosyltransferase-6 deficiency. Two transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
2528
RRID
AB_2765351
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from SW480 cells, using FUT6 Rabbit pAb (CAB3868) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human liver using FUT6 Rabbit pAb (CAB3868) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
