The FUT8 Antibody (CAB12933) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the FUT8 protein, this antibody enables the detection and analysis of FUT8 in various cell types, making it ideal for investigations in glycobiology and cancer research.FUT8, also known as α1,6-fucosyltransferase, plays a crucial role in the catalysis of core fucosylation, a type of protein glycosylation known to modulate various cellular processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
FUT8 Antibody
SKU:
CAB12933
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDGF, CDGF1, FUT8
Positive Sample:
A-549, Mouse kidney
Cellular Localization:
Golgi Apparatus, Golgi Stack Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
67kDa
Observed MW:
75kDa
This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
2530
RRID
AB_2759779
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FUT8 Rabbit pAb (CAB12933) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
