The FYB Antibody (CAB15997) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the FYB protein, this antibody enables accurate detection and analysis in a variety of cell types, facilitating studies in immunology and cancer research.FYB, also known as FYN-binding protein, is involved in multiple cellular processes, including T cell activation and migration. Its role in immune cell function makes it a critical target for investigating diseases such as cancer, autoimmune disorders, and inflammatory conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
FYB Antibody
SKU:
CAB15997
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FYB, ADAP, THC3, PRO0823, SLAP130, SLAP-130
Positive Sample:
Jurkat, Mouse spleen
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
85kDa
Observed MW:
120kDa
The protein encoded by this gene is an adapter for the FYN protein and LCP2 signaling cascades in T-cells. The encoded protein is involved in platelet activation and controls the expression of interleukin-2. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
2533
RRID
AB_2763437
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using FYB Rabbit pAb (CAB15997) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from Mouse spleen, using FYB Rabbit pAb (CAB15997) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
