The GADD45G Antibody (CAB10286) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications, enabling detection and analysis of GADD45G protein expression in various cell types.GADD45G, part of the growth arrest and DNA damage-inducible gene family, plays a crucial role in maintaining genomic stability and regulating cell cycle progression in response to environmental stressors. Its involvement in DNA repair mechanisms and cell growth control makes it a promising target for research in fields such as cancer biology, genotoxicity, and environmental toxicology.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GADD45G Antibody
SKU:
CAB10286
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CR6, DDIT2, GRP17, GADD45gamma, GADD45G
Positive Sample:
RAW264.7
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
17kDa
Observed MW:
17kDa
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta.
Purification Method
Affinity purification
Gene ID
10912
RRID
AB_2757828
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from RAW264.7 cells, using GADD45G Rabbit pAb (CAB10286) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5min.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using GADD45G Rabbit pAb (CAB10286) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using GADD45G Rabbit pAb (CAB10286) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse pancreas tissue using GADD45G Rabbit pAb (CAB10286) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
