The GAPVD1 Antibody (CAB17127) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the GAPVD1 protein, this antibody allows for the detection and analysis of GAPVD1 in various cell types, making it ideal for investigations in cell biology and cancer research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
GAPVD1 Antibody
SKU:
CAB17127
Size:
20μL, 100μL
Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RAP6, GAPEX5, GAPex-5, GAPVD1
Positive Sample:
mouse liver, mouse thymus
Cellular Localization:
Cytosol, Endosome, Plasma Membrane.
Calculated MW:
165kDa
Observed MW:
165kDa
Enables GTPase activating protein binding activity and guanyl-nucleotide exchange factor activity. Involved in regulation of protein transport. Located in cytosol and plasma membrane.
Purification Method
Affinity purification
Gene ID
26130
RRID
AB_2769573
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GAPVD1 Rabbit pAb (CAB17127) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of L929 cells using GAPVD1 Rabbit pAb (CAB17127) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
