The GBX2 Antibody (CAB14733) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot assays.GBX2 plays a crucial role in early embryonic development, specifically in the formation of the midbrain and hindbrain regions. Its aberrant expression has been linked to developmental disorders and certain types of cancer, making it a key target for research in developmental biology and oncology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GBX2 Antibody
SKU:
CAB14733
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GBX2
Positive Sample:
U-87MG, SH-SY5Y
Cellular Localization:
Nucleus.
Calculated MW:
37kDa
Observed MW:
34kDa
Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in regulation of nervous system development and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including branching involved in blood vessel morphogenesis; nervous system development; and neural crest cell migration. Predicted to be part of chromatin. Predicted to be active in nucleus.
Purification Method
Affinity purification
Gene ID
2637
RRID
AB_2761611
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GBX2 Rabbit pAb (CAB14733) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
