The GCN1L1 Antibody (CAB13075) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for Western blot applications.GCN1L1 is known to play a significant role in the integrated stress response pathway, helping cells adapt to various stressors like nutrient deprivation and viral infections.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
GCN1L1 Antibody
SKU:
CAB13075
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GCN1L, GCN1L1, PRIC295, L1
Positive Sample:
293T
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
293kDa
Observed MW:
293kDa
Enables RNA binding activity and cadherin binding activity. Predicted to be involved in cellular response to leucine starvation and positive regulation of transcription from RNA polymerase II promoter in response to stress. Located in cytosol.
Purification Method
Affinity purification
Gene ID
10985
RRID
AB_2759923
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and GCN1L1 knockout (KO) 293T(KO) cells, using [KO Validated] GCN1L1 Rabbit pAb (CAB13075) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
