The GEMIN7 Antibody (CAB15189) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the Gemin7 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in molecular biology and gene expression research.Gemin7 is known to play a critical role in the assembly of the Smn complex, which is essential for the biogenesis of small nuclear ribonucleoproteins (snRNPs) and the splicing of pre-mRNA.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
GEMIN7 Antibody
SKU:
CAB15189
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SIP3, GEMIN7
Positive Sample:
HeLa
Cellular Localization:
Cytoplasm, Nucleus, Gem, Nucleoplasm.
Calculated MW:
15kDa
Observed MW:
14kDa
The protein encoded by this gene is a component of the core SMN complex, which is required for pre-mRNA splicing in the nucleus. The encoded protein is found in the nucleoplasm, in nuclear "gems" (Gemini of Cajal bodies), and in the cytoplasm. Three transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
79760
RRID
AB_2762080
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using GEMIN7 Rabbit pAb (CAB15189) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
