General 1-Desamino 8D Arginine Vasopressin (DDAVP) ELISA Kit
The General-1 Desamino-8D-Arginine Vasopressin (DDAVP) ELISA Kit is a cutting-edge tool for accurately measuring DDAVP levels in various biological samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results for a wide range of research purposes. DDAVP, a synthetic hormone analog of vasopressin, plays a critical role in regulating water balance and blood pressure in the body. It is commonly used to treat conditions like diabetes insipidus, bedwetting, and certain bleeding disorders.
The accurate measurement of DDAVP levels is essential for monitoring treatment efficacy and understanding the underlying mechanisms of these conditions. By utilizing the General-1 Desamino-8D-Arginine Vasopressin (DDAVP) ELISA Kit, researchers can gain valuable insights into the physiological and therapeutic implications of DDAVP, paving the way for advancements in the field of endocrinology and related areas of study.
Product Name:
General 1-Desamino 8D Arginine Vasopressin (DDAVP) ELISA Kit
Product Code:
UNDL00026
Size:
96 Assays
Target:
DDAVP
Detection Method:
Competition
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
