The General Prostaglandin F2 Alpha (PGF2a) ELISA Kit is specifically designed for the accurate measurement of PGF2a levels in various biological samples such as serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a wide range of research purposes.Prostaglandin F2 Alpha is a potent lipid molecule that plays a crucial role in various physiological processes, including inflammation, smooth muscle contraction, and reproductive function. Its dysregulation has been linked to numerous health conditions, such as asthma, hypertension, and reproductive disorders.
Therefore, measuring PGF2a levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of potential therapeutic interventions.Overall, the General Prostaglandin F2 Alpha (PGF2a) ELISA Kit is a valuable tool for researchers seeking to accurately quantify PGF2a levels in biological samples and advance our understanding of the role of this important lipid mediator in health and disease.
Product Name:
General Prostaglandin F2 Alpha (PGF2a) ELISA Kit
Product Code:
UNDL00064
Size:
96 Assays
Target:
PGF2a
Synonyms:
PGF2A, PGF2-A, PG-F2a
Detection Method:
Competition
Range:
31.2-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
