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GenieHTS JC-10 Mitochondrial Membrane Potential Assay Kit

SKU:
ASIB009
Product Type:
Assay
Detection Method:
Fluorometric
Sample Type:
Cell Samples
Research Area:
Apoptosis
  • Figure 1. Fluorescence image overlays of HeLa cells stained using Assay Genie JC-10 Mitochondrial Membrane Potential assay kit acquired before (left) and after (right)the addition of CCCP, a potent mitochondrial decoupling reagent. Images were acquired using a 20X objective with GFP (green JC-10 monomer) and propidium iodide (orange JC-10 aggregate for healthy mitochondria) filter cubes.
  • Figure 2. Change in green/orange fluorescence ratio in cells treated with 1 or 10 μM CCCP, a potent mitochondrial decoupling reagent, for 4 hours. An increase in green/orange fluorescence is indicative of compromised or depolarized mitochondria. Fluorescence intensities were acquired using a plate reader (Ex/Em 490nm/525nm and 540nm/590nm).
  • Figure 3. Dose response curves of HeLa cells treated with staurosporine, an apoptosis-inducing protein kinase inhibitor. Measurements were acquired on the same cell populations 8 and 24 hours post- treatment. Fluorescence intensities were acquired using a plate reader (Ex/Em 490nm/525nm and 540nm/590nm).
$659
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Description

GenieHTS JC-10 Mitochondrial Membrane Potential Assay Kit

Mitochondrial membrane potential assay kit that uses JC-10, a membrane potential sensitive dye that is more soluble than JC-1.

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