The GET4 Antibody (CAB16190) is a high-quality antibody developed for reliable detection and analysis of target proteins. GET4, also known as TRC35, is involved in the targeting and insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane. This polyclonal antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for Western blot applications.By targeting the GET4 protein, researchers can gain valuable insights into the mechanisms underlying protein targeting and membrane insertion in cells. This antibody is essential for studying the role of GET4 in various cellular processes, including protein translocation and membrane biogenesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GET4 Antibody
SKU:
CAB16190
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CEE, CDG2Y, TRC35, CGI-20, C7orf20, GET4
Positive Sample:
SKOV3, A-549, HeLa, 293T, Mouse brain, Rat lung
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
37kDa
Observed MW:
36kDa
Enables chaperone binding activity. Involved in cytoplasmic sequestering of protein; maintenance of unfolded protein involved in ERAD pathway; and tail-anchored membrane protein insertion into ER membrane. Located in chromosome; cytosol; and nuclear lumen. Part of BAT3 complex.
Purification Method
Affinity purification
Gene ID
51608
RRID
AB_2763642
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GET4 Rabbit pAb (CAB16190) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
