The GFAP Monoclonal Antibody (CAB19058) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
This antibody is validated for use in WB, IHC-P, ELISA, IF-F, IF-P, mIHC applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GFAP Monoclonal Antibody
SKU:
CAB19058
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0206
Conjugate:
Unconjugated
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GFAP (P14136).
Tested Applications:
WBIHC-PELISAIF-FIF-PmIHC
Recommended Dilution:
WB
1:1000 - 1:2000
IF-F
1:200 - 1:2000
IF-P
1:200 - 1:2000
IHC-P
1:200 - 1:800
mIHC
1:200 - 1:800
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ALXDRD, GFAP
Positive Sample:
U-251 MG, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm.
Calculated MW:
50kDa
Observed MW:
50kDa
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method
Affinity purification
Gene ID
2670
RRID
AB_2862551
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using GFAP Rabbit mAb (CAB19058) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using GFAP Rabbit mAb (CAB19058)at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): HeLa Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using GFAP Rabbit mAb (CAB19058) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Human brain tissue using GFAP Rabbit mAb (CAB19058, dilution 1:1000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Rat brain tissue using GFAP Rabbit mAb (CAB19058, dilution 1:2000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
3D imaging of solvent-cleared mouse brain sections (at a thickness of 1 mm) using GFAP Rabbit mAb (CAB19058, diluted at a ratio of 1:200) . FDISCO JA11011 was used for sample clearing. We acknowledge Javis (Wuhan) Bio - Pharma Co., Ltd. in 3D imaging processing and kindly providing this image.
The multiplex IHC analysis on paraffin-embedded Mouse brain tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : NeuN Rabbit mAb (A19086, 1:2000) with TSA-TYR-520 (Green), GFAP Rabbit mAb (CAB19058, 1:500) with TSA-TYR-570 (Red), and TMEM119 Rabbit mAb (A27143, 1:600) with TSA-TYR-690 (Yellow). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.
The multiplex IHC analysis on paraffin-embedded Rat brain tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : NeuN Rabbit mAb (A19086, 1:2000) with TSA-TYR-520 (Green), GFAP Rabbit mAb (CAB19058, 1:500) with TSA-TYR-570 (Red), and TMEM119 Rabbit mAb (A27143, 1:600) with TSA-TYR-690 (Yellow). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.
