GFP Monoclonal Antibody is a premium monoclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IF, FC, IP, this antibody ensures consistent, reproducible results across multiple experimental platforms. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
GFP Monoclonal Antibody (MACO0665)
SKU:
MACO0665
Size:
50μg
Isotype:
IgG2b
Host Species:
Mouse
Reactivity:
N/A
Immunogen:
Recombinant GFP Protein
Form:
liquid
Tested Applications:
ELISAWBIFFCIP
Recommended Dilution:
WB 1:50000-1:6400000, IF 1:50-1:200, FC 1:100-1:300, IP 1µl-2µl
Western Blot Positive WB detected in: 50ng recombinant protein All lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 KDa Exposure time:5min
Western Blot Positive WB detected in: Recombinant protein at 25ng, 12.5ng, 6.25ng, 3.125ng, 1.56ng, 0.78ng, 0.39ng, 0.195ng All lanes: GFP antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 KDa Exposure time:5min
Western Blot Positive WB detected in: 1-4 lanes:Recombinant proteins with GFP tag for 50ng; 5 lane: 293F whole cell lysate transfected with GFP for 5µg All lanes GFP antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectively Observed band size: 32,32,50,32,32 KDa Exposure time:1min
Immunofluorescence staining of 293F cells transfected with GFP with MACO0665 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).
Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with MACO0665 at 1:200. The cells were fixed in 70% ethanol at 4°C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C.
Overlay histogram showing 293F cells transfected with GFP stained with MACO0665 (red line) at 1:200. The cells were fixed in 70% ethanol at 4°C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunoprecipitating GFP in 293F whole cell lysate transfected with GFP Lane 1: Mouse control IgG2b instead of MACO0665 in 293F whole cell lysate transfected with GFP Lane 2: MACO0665 (4µg) + 293F whole cell lysate transfected with GFP (500µg) Lane 3: 293F whole cell lysate transfected with GFP (5µg) For western blotting, the blot was detected with MACO0665 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
