The GHRH Monoclonal Antibody (CAB23232) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, developed by AssayGenie, specifically targets GHRH, a key regulator of growth hormone secretion and other physiological processes.This antibody, produced through advanced monoclonal antibody technology, is highly specific and sensitive for detecting GHRH in various samples, including tissues and cell lysates. It has been validated for use in a range of applications, including Western blot, immunofluorescence, and immunohistochemistry, providing researchers with a versatile tool for their experiments.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
GHRH Monoclonal Antibody
SKU:
CAB23232
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC59237
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GRF, INN, GHRF, GHRH
Positive Sample:
293T transfected with GHRH
Cellular Localization:
Secreted.
Calculated MW:
12kDa
Observed MW:
17kDa
This gene encodes a member of the glucagon family of proteins. The encoded preproprotein is produced in the hypothalamus and cleaved to generate the mature factor, known as somatoliberin, which acts to stimulate growth hormone release from the pituitary gland. Variant receptors for somatoliberin have been found in several types of tumors, and antagonists of these receptors can inhibit the growth of the tumors. Defects in this gene are a cause of dwarfism, while hypersecretion of the encoded protein is a cause of gigantism. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Purification Method
Affinity purification
Gene ID
2691
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with GHRH using GHRH Rabbit mAb (CAB23232) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
