The GIPR Antibody (CAB9816) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, shows high reactivity with human samples and has been validated for use in various applications, including Western blotting.The GIPR protein plays a crucial role in glucose metabolism and is a potential target for therapeutic interventions in metabolic disorders such as diabetes and obesity. By binding specifically to GIPR, this antibody enables the detection and analysis of GIPR expression in different cell types, making it an essential tool for studies in metabolism, endocrinology, and related fields.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GIPR Antibody
SKU:
CAB9816
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PGQTL2, GIPR
Positive Sample:
K-562, Mouse liver, Rat heart, Rat brain
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
53kDa
Observed MW:
45kDa
This gene encodes a G-protein coupled receptor for gastric inhibitory polypeptide (GIP), which was originally identified as an activity in gut extracts that inhibited gastric acid secretion and gastrin release, but subsequently was demonstrated to stimulate insulin release in the presence of elevated glucose. Mice lacking this gene exhibit higher blood glucose levels with impaired initial insulin response after oral glucose load. Defect in this gene thus may contribute to the pathogenesis of diabetes.
Purification Method
Affinity purification
Gene ID
2696
RRID
AB_2769606
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GIPR Rabbit pAb (CAB9816) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using GIPR Rabbit pAb (CAB9816) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
