The GLYCTK Antibody (CAB7852) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity with glycerate kinase in various biological samples, particularly in human tissues.Utilizing this antibody in Western blot applications allows for the accurate detection and analysis of glycerate kinase protein expression levels in different cell types and tissues. Its reliability and performance make it a valuable tool for researchers investigating the metabolic pathways that play a crucial role in various physiological processes.Glycerate kinase is a key enzyme that catalyzes the conversion of glycerate to 2-phosphoglycerate, playing a significant role in energy metabolism and amino acid synthesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GLYCTK Antibody
SKU:
CAB7852
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HBEBP2, HBEBP4, HBeAgBP4A, GLYCTK
Positive Sample:
Mouse liver, Mouse kidney, Rat liver, Rat kidney
Cellular Localization:
Cytoplasm, Cytoplasm, Mitochondrion.
Calculated MW:
55kDa
Observed MW:
55kDa
This locus encodes a member of the glycerate kinase type-2 family. The encoded enzyme catalyzes the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. Decreased activity of the encoded enzyme may be associated with the disease D-glyceric aciduria. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
132158
RRID
AB_2769623
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GLYCTK Rabbit pAb (CAB7852) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
