Description
GNAS Antibody (CAB2732)
The GNAS Antibody (CAB2732) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity towards human samples and has been validated for use in Western blot applications. By binding to the GNAS protein, this antibody enables accurate detection and analysis in various cell types, making it an excellent choice for studies in molecular biology, endocrinology, and signal transduction research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
| Product Name: | GNAS Antibody |
| SKU: | CAB2732 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | MGCL GNSK TEDQ RNEE KAQR EANK KIEK QLQK DKQV YRAT HRLL LLGA GESG KSTI VKQM RILH VNGF NGEG GEED PQAA RSNS DGEK ATKV QDIK NNLK | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | AHO, GSA, GSP, POH, GPSA, NESP, SCG6, SgVI, GNAS1, PITA3, C20orf45, GNAS |
| Positive Sample: | NCI-H460, SW620, Mouse skeletal muscle, Mouse brain, Mouse heart |
| Cellular Localization: | Cell Membrane By Similarity; Lipid-Anchor By Similarity. |
| Calculated MW: | 28kDa/44kDa/45kDa/77kDa/109kDa/111kDa |
| Observed MW: | 43kDa |
This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
| Purification Method | Affinity purification |
| Gene ID | 2778 |
| RRID | AB_2764582 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using GNAS Rabbit pAb (CAB2732) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. |
