The GNB5 Antibody (CAB4447) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the GNB5 protein, allowing for accurate detection and analysis in a variety of cell types.GNB5 plays a crucial role in many cellular processes, including receptor signaling, intracellular trafficking, and cytoskeletal dynamics. Its involvement in these pathways makes it a key target for research in fields such as cell biology, neurobiology, and cancer biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GNB5 Antibody
SKU:
CAB4447
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GB5, HG2E, IDDCA, LADCI, gbeta5, GNB5
Positive Sample:
Jurkat, HL-60, 293T, Mouse brain, Rat brain
Cellular Localization:
Cytoplasm, Cytosol, Nucleus, Presynapse.
Calculated MW:
44kDa
Observed MW:
39kDa
Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist.
Purification Method
Affinity purification
Gene ID
10681
RRID
AB_2765681
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GNB5 Rabbit pAb (CAB4447) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 60s.
