The GOLPH3 Antibody (CAB13121) is a high-quality antibody developed for reliable detection and analysis of target proteins. GOLPH3 is a highly conserved protein located primarily in the Golgi apparatus, where it plays a key role in membrane trafficking and protein glycosylation.This polyclonal antibody, raised in rabbits, specifically targets GOLPH3 and is validated for use in various applications, including Western blotting and immunofluorescence. Its high reactivity with human samples makes it an ideal choice for studies involving GOLPH3 in cancer research, as dysregulation of GOLPH3 has been implicated in tumor growth, invasion, and metastasis.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GOLPH3 Antibody
SKU:
CAB13121
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
Purification Method
Affinity purification
Gene ID
64083
RRID
AB_2759970
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GOLPH3 Rabbit pAb (CAB13121) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Immunofluorescence analysis of L929 cells using GOLPH3 Rabbit pAb (CAB13121) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
