The GPNMB Antibody (CAB14270) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, displays high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the GPNMB protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in immunology and cancer research.GPNMB, also known as a melanoma-associated antigen, has been implicated in various diseases, including cancer and inflammatory disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GPNMB Antibody
SKU:
CAB14270
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NMB, HGFIN, PLCA3, GPNMB
Positive Sample:
SK-MEL-28, Rat eye
Cellular Localization:
Cell Membrane, Melanosome, Single-Pass Type I Membrane Protein.
Calculated MW:
64kDa
Observed MW:
64kDa/90-120kDa
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
10457
RRID
AB_2761133
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from SK-MEL-28 cells, using GPNMB Rabbit pAb (CAB14270) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Rat eye, using GPNMB Rabbit pAb (CAB14270) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
