The GPR22 Antibody (CAB18313) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the GPR22 protein, this antibody enables accurate detection and analysis in multiple cell types, making it ideal for investigations in pharmacology and neurobiology.GPR22, also recognized as an orphan GPCR, plays a vital role in modulating cell signaling pathways and neurotransmitter release, making it a promising target for drug development in neurological disorders and psychiatric conditions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GPR22 Antibody
SKU:
CAB18313
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GPR22
Positive Sample:
U-87MG, U-251MG, SH-SY5Y, HepG2, Mouse heart, Mouse brain, Rat heart, Rat brain
Cellular Localization:
Plasma Membrane.
Calculated MW:
49kDa
Observed MW:
55kDa
This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein.
Purification Method
Affinity purification
Gene ID
2845
RRID
AB_2862085
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GPR22 Rabbit pAb (CAB18313) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of L929 cells using GPR22 Rabbit pAb (CAB18313) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
