The GPT Antibody (CAB2814) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it an ideal choice for Western blotting applications.GPT is an essential enzyme involved in amino acid metabolism and plays a key role in the conversion of alanine and α-ketoglutarate to pyruvate and glutamate. Abnormal levels of GPT in the blood are indicative of liver damage and are commonly used as a biomarker for liver function in diagnostic tests.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GPT Antibody
SKU:
CAB2814
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ALT, AAT1, ALT1, GPT1, SGPT, GPT
Positive Sample:
Mouse liver, Mouse lung, Mouse intestine, Rat liver, Rat heart
Cellular Localization:
Cytoplasm.
Calculated MW:
55kDa
Observed MW:
55kDa
This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
Purification Method
Affinity purification
Gene ID
2875
RRID
AB_2764652
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GPT Rabbit pAb (CAB2814) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
