Description
Granulin Monoclonal Antibody (CAB23230)
The Granulin Monoclonal Antibody (CAB23230) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, created using cutting-edge technology, specifically targets granulin and is highly reactive with human samples.Granulin is known to play a key role in cancer development and progression, making it a significant target for studies in oncology research. By utilizing this antibody in Western blot applications, researchers can effectively detect and analyze granulin levels in different cell types, providing valuable insights into its function and potential implications in cancer biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
| Product Name: | Granulin Monoclonal Antibody |
| SKU: | CAB23230 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse |
| Clone Number: | ARC59451 |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | SCEK EVVS AQPA TFLA RSPH VGVK DVEC GEGH FCHD NQTC CRDN RQGW ACCP YRQG VCCA DRRH CCPA GFRC AARG TKCL RREA PRWD APLR DPAL RQLL | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | GEP, GP88, PEPI, PGRN, CLN11, PCDGF, Granulin |
| Positive Sample: | HeLa, Mouse liver |
| Cellular Localization: | Secreted, Lysosome. |
| Calculated MW: | 64kDa |
| Observed MW: | 75-88kDa |
Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
| Purification Method | Affinity purification |
| Gene ID | 2896 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from HeLa cells, using Granulin Rabbit mAb (CAB23230) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s. |
 | Western blot analysis of lysates from Mouse liver, using Granulin Rabbit mAb (CAB23230) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s. |
