The Granzyme B Antibody (CAB2557) is a high-quality antibody developed for reliable detection and analysis of target proteins. Granzyme B is a serine protease found in cytotoxic lymphocytes, and is involved in inducing apoptosis in target cells.This antibody, raised in rabbits, is highly specific to Granzyme B and has been validated for use in various applications, including Western blot and immunohistochemistry. Its high reactivity with human samples makes it ideal for studies in immunology, cancer research, and autoimmune diseases.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Granzyme B Antibody
SKU:
CAB2557
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis.
Purification Method
Affinity purification
Gene ID
3002
RRID
AB_2764445
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Granzyme B Rabbit pAb (CAB2557) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Granzyme B Rabbit pAb (CAB2557) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
