The GRHL2 Polyclonal Antibody (CAB24468) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through immunization of rabbits, has been optimized for use in Western blot applications and is highly reactive with human samples.GRHL2, also known as grainyhead-like 2, is involved in the regulation of gene expression related to cell adhesion, epithelial barrier function, and epithelial-to-mesenchymal transition. Dysregulation of GRHL2 has been implicated in numerous cancers, making it a promising target for further investigation in cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
GRHL2 Polyclonal Antibody
SKU:
CAB24468
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GRHL2, BOM, DFNA28, ECTDS, TFCP2L3, grainyhead-like protein 2 homolog
Positive Sample:
T-47D, Rat lung
Cellular Localization:
Membrane, Nucleus.
Calculated MW:
69kDa/71kDa
Observed MW:
71kDa
The protein encoded by this gene is a transcription factor that can act as a homodimer or as a heterodimer with either GRHL1 or GRHL3. Defects in this gene are a cause of non-syndromic sensorineural deafness autosomal dominant type 28 (DFNA28).
Purification Method
Affinity purification
Gene ID
79977
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from T-47D cells, using GRHL2 Rabbit pAb (CAB24468) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from Rat lung, using GRHL2 Rabbit pAb (CAB24468) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
