The GRID2 Polyclonal Antibody (CAB19314) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbit serum, is highly specific to human samples and has been validated for use in various applications, including immunofluorescence and immunohistochemistry.GRID2, a member of the glutamate receptor family, is essential for regulating synaptic strength and neural connectivity in the brain. Dysregulation of GRID2 function has been implicated in various neurological disorders, including ataxia and schizophrenia.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GRID2 Polyclonal Antibody
SKU:
CAB19314
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GluD2, SCAR18, GRID2
Positive Sample:
Mouse brian
Cellular Localization:
Dendritic Spine, Glutamatergic Synapse, Parallel Fiber To Purkinje Cell Synapse, Plasma Membrane, Postsynaptic Density Membrane, Postsynaptic Membrane, Synapse.
Calculated MW:
113kDa
Observed MW:
113kDa
The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans.
Purification Method
Affinity purification
Gene ID
2895
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brian, using GRID2 Rabbit pAb (CAB19314) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of C6 cells using GRID2 Rabbit pAb (CAB19314) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using GRID2 Rabbit pAb (CAB19314) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
