Description
GRK1 Colorimetric Cell-Based ELISA Kit
The GRK1 Colorimetric Cell-Based ELISA Kit is an innovative tool designed for the detection of G protein-coupled receptor kinase 1 (GRK1) levels in cell lysates. This kit offers high sensitivity and specificity, providing accurate and reproducible results for research purposes.GRK1 is a key enzyme involved in regulating the phosphorylation and desensitization of G protein-coupled receptors, impacting signaling pathways and cellular responses. Dysregulation of GRK1 has been linked to various diseases, including cardiovascular disorders, neurodegenerative diseases, and metabolic conditions.
With the GRK1 Colorimetric Cell-Based ELISA Kit, researchers can effectively study the role of GRK1 in disease pathogenesis and potential therapeutic interventions. This kit is suitable for a wide range of cell-based assays, making it a valuable tool for scientific studies in the field of cellular signaling and drug development.
| Product Name: | GRK1 Colorimetric Cell-Based ELISA Kit |
| Product Code: | CBCAB00681 |
| ELISA Type: | Cell-Based |
| Target: | GRK1 |
| Reactivity: | Human, Mouse, Rat |
| Dynamic Range: | > 5000 Cells |
| Detection Method: | Colorimetric 450 nmStorage/Stability:4°C/6 Months |
| Format: | 96-Well Microplate |
The GRK1 Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect GRK1 protein expression profile in cells. The kit can be used for measuring the relative amounts of GRK1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on GRK1.
Qualitative determination of GRK1 concentration is achieved by an indirect ELISA format. In essence, GRK1 is captured by GRK1-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:
| 1. | A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. |
| 2. | Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. |
| Database Information: | Gene ID: 6011, UniProt ID: Q15835, OMIM: 180381/258100, Unigene: Hs.103501/Hs.721727 |
| Gene Symbol: | GRK1 |
| Sub Type: | None |
| UniProt Protein Function: | GRK1: a Ser/Thr of the GRK family. Phosphorylates and inactivates photon-activated rhodopsin. LOF mutations lead to type 2 Oguchi disease, a form of night blindness. A link between RHOK and retinitis pigmentosa is now thought to be unlikely |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.14; Kinase, protein; AGC group; GRK family; GRK subfamily Chromosomal Location of Human Ortholog: 13q34 Molecular Function:G-protein coupled receptor kinase activity; rhodopsin kinase activity; ATP binding; protein kinase activity Biological Process: rhodopsin mediated signaling; post-embryonic retina morphogenesis in camera-type eye; phototransduction, visible light; regulation of rhodopsin mediated signaling; visual perception; protein amino acid autophosphorylation; photoreceptor cell morphogenesis; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of phosphorylation; negative regulation of apoptosis Disease: Oguchi Disease 2 |
| NCBI Summary: | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15835 |
| NCBI GenInfo Identifier: | 2833269 |
| NCBI Gene ID: | 6011 |
| NCBI Accession: | Q15835.1 |
| UniProt Secondary Accession: | Q15835,Q53X14, |
| UniProt Related Accession: | Q15835 |
| Molecular Weight: | 563 |
| NCBI Full Name: | Rhodopsin kinase |
| NCBI Synonym Full Names: | G protein-coupled receptor kinase 1 |
| NCBI Official Symbol: | GRK1Â Â |
| NCBI Official Synonym Symbols: | RK; RHOK; GPRK1Â Â |
| NCBI Protein Information: | rhodopsin kinase |
| UniProt Protein Name: | Rhodopsin kinase |
| UniProt Synonym Protein Names: | G protein-coupled receptor kinase 1 |
| UniProt Gene Name: | GRK1Â Â |
| UniProt Entry Name: | RK_HUMAN |
| Component | Quantity |
| 96-Well Cell Culture Clear-Bottom Microplate | 2 plates |
| 10X TBS | 24 mL |
| Quenching Buffer | 24 mL |
| Blocking Buffer | 50 mL |
| 15X Wash Buffer | 50 mL |
| Primary Antibody Diluent | 12 mL |
| 100x Anti-Phospho Target Antibody | 60 µL |
| 100x Anti-Target Antibody | 60 µL |
| Anti-GAPDH Antibody | 60 µL |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12 mL |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12 mL |
| SDS Solution | 12 mL |
| Stop Solution | 24 mL |
| Ready-to-Use Substrate | 12 mL |
| Crystal Violet Solution | 12 mL |
| Adhesive Plate Seals | 2 seals |
The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing ≥1 ml
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
- Orbital shaker (optional)
- Deionized or sterile water
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1. | Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells. |
| 2. | Incubate the cells for overnight at 37°C, 5% CO2. |
| 3. | Treat the cells as desired. |
| 4. | Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice. |
| 5. | Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells. |
| 6. | Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week. |
| 7. | Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature. |
| 8. | Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time. |
| 9. | Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature. |
| 10. | Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time. |
| 11. | Add 50 µL of 1x primary antibodies (Anti-GRK1 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature. |
| 12. | Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time. |
| 13. | Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature. |
| 14. | Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time. |
| 15. | Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark. |
| 16. | Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader. |
(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)
