The GRPR Antibody (CAB2940) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has been shown to have high reactivity with human samples, making it suitable for use in Western blotting applications.By specifically binding to the GRPR protein, this antibody enables researchers to accurately detect and analyze GRPR expression in different cell types, providing valuable insights for studies in neuroscience, oncology, and endocrinology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GRPR Antibody
SKU:
CAB2940
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BB2, BB2R, BRS2, GRPR
Positive Sample:
U-87MG, SGC-7901, A-549, MCF7, Mouse brain, Mouse pancreas, Rat brain
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
43kDa
Observed MW:
43kDa
Gastrin-releasing peptide (GRP) regulates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation and is a potent mitogen for neoplastic tissues. The effects of GRP are mediated through the gastrin-releasing peptide receptor. This receptor is a glycosylated, 7-transmembrane G-protein coupled receptor that activates the phospholipase C signaling pathway. The receptor is aberrantly expressed in numerous cancers such as those of the lung, colon, and prostate. An individual with autism and multiple exostoses was found to have a balanced translocation between chromosome 8 and a chromosome X breakpoint located within the gastrin-releasing peptide receptor gene.
Purification Method
Affinity purification
Gene ID
2925
RRID
AB_2764754
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GRPR Rabbit pAb (CAB2940) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
