GST Monoclonal Antibody is a premium monoclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IF, FC, IP, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with All samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ul format to meet your experimental needs. For optimal performance, store at Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
GST Monoclonal Antibody (MACO0667)
SKU:
MACO0667
Size:
50μl
Isotype:
IgG2b
Host Species:
Mouse
Reactivity:
All
Immunogen:
Recombinant GST Protein
Form:
Liquid
Tested Applications:
ELISAWBIFFCIP
Recommended Dilution:
WB 1:10000-1:640000, IF 1:100-1:300, FC 1:100-1:300, IP 1ul-5ul
`Western Blot Positive WB detected in: 25ng Recombinant protein with GST tag from E.coli All lanes: GST antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32.6, 30.1, 29.5, 37.4, 30.4, 60.5, 38.1, 61.6 KDa Observed band size: 31, 30, 31, 37, 30, 60, 37, 61 KDa Exposure time：5min
`Western Blot Positive WB detected in: 50ng Recombinant protein with GST tag from E.coli All lanes: GST antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 26, 102.9, 75.6 KDa Observed band size: 26, 103, 76 KDa Exposure time：1min
`Western Blot Positive WB detected in: Recombinant protein with GST tag from E.coli at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng All lanes: GST antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa Exposure time：5min
`Western Blot Positive WB detected in: 25ng recombinant protein with GST tag from E.coli GST antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa Exposure time：5min
Immunofluorescence staining of 293F transfected cells with MACO0667 at 1:94.5, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunoprecipitating GST in transfected-293F whole cell lysate Lane 1: Mouse control IgG instead of MACO0667 in 293F cell lysate Transfected with GST Lane 2: MACO0667 (5µg) + Transfected-293F whole cell lysate (500µg) Lane 3: Transfected-293F whole cell lysate (10µg) For western blotting, the blot was detected with MACO0667 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
Overlay Peak curve showing 293F cells transfected with GST stained with MACO0667 (red line) at 1:189. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
