The GSTO1 Antibody (CAB15354) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to GSTO1, researchers can accurately detect and analyze this crucial enzyme in a variety of cell types, making it ideal for studies in oxidative stress, drug metabolism, and cancer research.GSTO1 is known for its role in cellular protection against oxidative damage and its involvement in the metabolism of certain chemotherapeutic agents.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GSTO1 Antibody
SKU:
CAB15354
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P28, SPG-R, GSTO 1-1, GSTTLp28, HEL-S-21, GSTO1
Positive Sample:
LO2, BxPC-3, HT-29, HeLa, Mouse heart, Rat liver
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
28kDa
Observed MW:
27kDa
The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
9446
RRID
AB_2762256
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GSTO1 Rabbit pAb (CAB15354) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
