The GTF2E2 Antibody (CAB8732) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to the GTF2E2 protein, this antibody enables the detection and analysis of GTF2E2 in a variety of cell types, making it ideal for investigations in the fields of molecular biology and gene regulation.GTF2E2, also known as general transcription factor IIE subunit 2, plays a crucial role in the initiation of transcription by RNA polymerase II. Its functions are essential for the accurate and efficient production of mRNA, making it a key player in gene expression processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GTF2E2 Antibody
SKU:
CAB8732
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FE, TTD6, TF2E2, TFIIE-B, GTF2E2
Positive Sample:
Raji, Mouse testis
Cellular Localization:
Nucleus.
Calculated MW:
33kDa
Observed MW:
33kDa
The general transcription factor IIE (TFIIE) is part of the RNA polymerase II transcription initiation complex, recruiting TFIIH and being essential for promoter clearance by RNA polymerase II. TFIIE is a heterodimer (and sometimes heterotetramer) of alpha and beta subunits. The protein encoded by this gene represents the beta subunit of TFIIE.
Purification Method
Affinity purification
Gene ID
2961
RRID
AB_2769711
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GTF2E2 Rabbit pAb (CAB8732) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
