The GTF2H3 Antibody (CAB7188) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the GTF2H3 protein, this antibody enables the detection and analysis of GTF2H3 in various cell types, making it an essential component for studies in molecular biology and cancer research.GTF2H3 is an essential subunit of the general transcription factor IIH complex, which plays a crucial role in the initiation of transcription and DNA repair mechanisms.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
GTF2H3 Antibody
SKU:
CAB7188
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P34, BTF2, TFB4, TFIIH, GTF2H3
Positive Sample:
HL-60, HT-29, MCF-7, Jurkat
Cellular Localization:
Nucleus.
Calculated MW:
34kDa
Observed MW:
36kDa
This gene encodes a member of the TFB4 family. The encoded protein is a subunit of the core-TFIIH basal transcription factor and localizes to the nucleus. The encoded protein is involved in RNA transcription by RNA polymerase II and nucleotide excision repair and associates with the Cdk-activating kinase complex. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 14.
Purification Method
Affinity purification
Gene ID
2967
RRID
AB_2767738
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GTF2H3 Rabbit pAb (CAB7188) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation analysis of 200 μg extracts of HT-29 cells using 1 μg GTF2H3 antibody (CAB7188). Western blot was performed from the immunoprecipitate using GTF2H3 antibody (CAB7188) at a dilution of 1:1000.
