The GTF3C3 Antibody (CAB18405) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the GTF3C3 protein, this antibody enables precise detection and analysis in various cell types, facilitating in-depth research in molecular biology and gene expression regulation.GTF3C3 is a critical factor in the initiation of transcription by RNA polymerase III, playing a key role in the synthesis of small nuclear RNAs and other non-coding RNAs essential for cellular function.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GTF3C3 Antibody
SKU:
CAB18405
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TFIIIC102, TFIIICgamma, TFiiiC2-102, GTF3C3
Positive Sample:
HepG2, HeLa, 293T, RAW264.7, NIH/3T3, Mouse lung
Cellular Localization:
Nuclear Membrane, Nucleolus, Nucleoplasm.
Calculated MW:
101kDa
Observed MW:
101kDa
The protein encoded by this gene is part of the TFIIIC2 complex, which binds to the promoters of small nuclear and cytoplasmic RNA genes in order to recruit RNA polymerase III. The TFIIIC2 complex is composed of six subunits. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
9330
RRID
AB_2862172
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GTF3C3 Rabbit pAb (CAB18405) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using GTF3C3 Rabbit pAb (CAB18405) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of H9C2 cells using GTF3C3 Rabbit pAb (CAB18405) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using GTF3C3 Rabbit pAb (CAB18405) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
